Estrogen Metabolism Test

About this test

What This Test Measures

GetTested’s Estrogen Metabolism Test measures parent estrogens along with a range of metabolites that reflect how estrogen is processed in the body.

E2 (estradiol), E1 (estrone), and E3 (estriol) represent the primary forms of estrogen. These are further converted into metabolites through pathways such as 2-, 4-, and 16α-hydroxylation, which influence how estrogen is broken down.

Methylated metabolites (2-MeO and 4-MeO forms) reflect how these compounds are further processed and cleared from the body.

The test also includes BPA, an environmental compound that may interact with hormonal pathways, and creatinine, which is used to normalize urine concentrations for more accurate interpretation.

By measuring these markers together, the test provides a detailed view of estrogen metabolism and how these processes are functioning.

How It Works

You collect your sample at home using the instructions provided in the kit. The dried urine sample is then sent to a certified laboratory for analysis, and your results are delivered digitally once testing is complete.

Dried Urine Collection

The test uses a dried urine sample collected at home.

This method allows for convenient sampling without the need for blood collection and is commonly used to assess hormone metabolites. Once collected, the sample is dried and sent to the laboratory for analysis.

Who Should Consider This Test

This test is relevant for individuals who want a deeper understanding of estrogen metabolism and how estrogen is processed in the body.

It may be particularly useful in cases of hormone-related symptoms, when exploring hormonal balance, or when seeking a more detailed assessment beyond standard hormone testing. It can also be used to better understand how environmental and metabolic factors may interact with hormone pathways.

Understanding CLIA Certification

Your sample is analyzed in a CLIA-certified laboratory, ensuring that testing is performed according to established U.S. quality standards using validated methods.

Biomarkers included

• Estradiol (E2): Primary estrogens — Estradiol is the most biologically active form of estrogen in the body. It plays a central role in reproductive health, regulating the menstrual cycle, ovulation, and fertility. Estradiol also affects bone density, cardiovascular health, brain function, and metabolism. Levels vary throughout the menstrual cycle and across different life stages such as puberty, pregnancy, and menopause. Measuring e
• Estriol (E3): Primary estrogens — Estriol is a naturally occurring estrogen and the weakest of the three primary estrogens. It is produced in small amounts in non-pregnant individuals but becomes the dominant estrogen during pregnancy, as it is generated through interactions between the placenta and the fetus. Estriol reflects aspects of estrogen metabolism and hormone turnover. Measuring estriol can help provide a broader picture
• Estrone (E1): Primary estrogens — Estrone is one of the three main forms of estrogen produced in the body. It is synthesized primarily from androgens in fat tissue and becomes the dominant estrogen after menopause. Estrone can be converted into estradiol or further metabolized into various estrogen metabolites. Measuring estrone helps provide insight into overall estrogen production and balance, particularly in relation to age, bo
• 2-Hydroxyestrone: Hydroxylated estrogen metabolites — 2-Hydroxyestrone is produced when estrone undergoes hydroxylation at the 2-position during estrogen metabolism. It is part of the 2-hydroxylation pathway, one of the main metabolic routes for estrogen processing. This metabolite can be further converted into 2-methoxyestrone through methylation reactions. Measuring 2-hydroxyestrone provides insight into estrogen metabolism patterns and how estrone
• 16α-Hydroxyestrone: Hydroxylated estrogen metabolites — 16α-Hydroxyestrone is a metabolite produced when estrone is metabolized through the 16α-hydroxylation pathway. This pathway represents one of the main routes of estrogen metabolism alongside the 2- and 4-hydroxylation pathways. Unlike some other metabolites, 16α-hydroxyestrone retains estrogenic activity and can interact with estrogen receptors. Measuring this marker helps provide insight into the
• 2-Hydroxyestradiol: Hydroxylated estrogen metabolites — 2-Hydroxyisobutyric Acid is a metabolite associated with exposure to methyl tert-butyl ether (MTBE), a fuel additive used in gasoline that may contribute to environmental pollution and air contamination.
• 4-Hydroxyestrone: Hydroxylated estrogen metabolites — 4-Hydroxyestrone is generated when estrone undergoes hydroxylation at the 4-position during estrogen metabolism. Like other hydroxylated metabolites, it represents an intermediate step before further methylation or elimination. Monitoring 4-hydroxyestrone provides information about how estrone is metabolized and which pathways are active in estrogen breakdown.
• 4-Hydroxyestradiol: Hydroxylated estrogen metabolites — 4-Hydroxyestradiol is a metabolite formed when estradiol is metabolized through the 4-hydroxylation pathway. This pathway represents another route by which the body processes estrogens before further modification and elimination. The metabolite can be converted into methylated forms such as 4-methoxyestradiol. Measuring 4-hydroxyestradiol helps evaluate how estradiol is being processed through dif
• 2-Methoxyestradiol: Methylated estrogen metabolites — 2-Methoxyestradiol is a methylated metabolite formed when 2-hydroxyestradiol undergoes a methylation reaction, typically involving the enzyme catechol-O-methyltransferase (COMT). This process represents a secondary step in estrogen metabolism that helps convert hydroxylated metabolites into more stable forms for elimination. Measuring 2-methoxyestradiol provides information about the efficiency of
• 2-Methoxyestrone: Methylated estrogen metabolites — 2-Methoxyestrone is produced when 2-hydroxyestrone is methylated as part of phase II estrogen metabolism. Methylation reactions help the body further process estrogen metabolites so they can be more easily excreted. Monitoring 2-methoxyestrone can help assess how effectively the body is carrying out this secondary detoxification step within estrogen metabolism.
• 4-Methoxyestrone: Methylated estrogen metabolites — 4-Methoxyestrone is a methylated estrogen metabolite formed when 4-hydroxyestrone undergoes a methylation reaction during phase II estrogen metabolism. This step is typically catalyzed by the enzyme catechol-O-methyltransferase (COMT), which helps convert reactive hydroxylated estrogen metabolites into more stable forms that can be eliminated from the body. Measuring 4-methoxyestrone provides insi
• 4-Methoxyestradiol: Methylated estrogen metabolites — 4-Methoxyestradiol is a methylated metabolite formed when 4-hydroxyestradiol undergoes methylation. This reaction is part of the body’s process of converting reactive hydroxylated estrogen metabolites into more stable compounds that can be cleared from the body. Measuring 4-methoxyestradiol helps provide insight into how the 4-hydroxylation pathway metabolites are being further processed and elimi
• Bisphenol A (BPA): Environmental exposure marker — Bisphenol A is an industrial chemical commonly used in the production of certain plastics and resins, including polycarbonate plastics and epoxy coatings found in food containers and packaging materials. BPA is considered an endocrine-active compound because it can interact with estrogen receptors and influence hormone signaling pathways. Measuring BPA levels in urine can help assess environmental
• Creatinine: Normalization marker — Creatinine is a waste product formed in muscles from the normal breakdown of creatine. It is released into the bloodstream and filtered by the kidneys, then excreted in urine. Because creatinine levels are influenced by how efficiently the kidneys filter blood, it is commonly used to assess kidney function. Elevated creatinine may indicate reduced kidney filtration or kidney disease, while low lev

How to prepare

Detailed instructions are provided with your kit. Specific guidance on diet, supplements, medications, and alcohol intake to ensure accurate results.

Frequently asked questions

What specifically does this estrogen metabolite test measure?

This comprehensive estrogen test measures parent estrogens (E1, E2, E3), their crucial metabolites (2-OH E2, 2-OH E1, 4-OH E2, 4-OH E1, 16aOH E1, 2-MeO E2, 2-MeO E1, 4-MeO E2, 4-MeO E1), Bisphenol A (BPA), and Creatinine (Crtn). This detailed analysis provides insights into your estrogen metabolism pathways and estrogen detoxification. This is an advanced 2-OH E1 E2 E3 test.

How does this dried urine hormone test help understand hormone balance?

By analyzing a wide range of estrogen metabolites, this test provides a snapshot of how your body produces, processes, and eliminates estrogens. Imbalances in these pathways can lead to high estrogen symptoms or estrogen dominance. Understanding these patterns is key to achieving natural hormone balance, making it an excellent hormone balance test at home.

Why is BPA included in this hormone health urine test?

Bisphenol A (BPA) is an environmental toxin known to interfere with hormone function and estrogen metabolism. Including BPA in this test allows for the assessment of potential BPA hormone disruption and its impact on your estrogen detoxification, providing a more complete understanding of factors affecting your hormone health.

Can men take this estrogen metabolism test?

Yes. Estrogen metabolism is relevant in both women and men, as estrogen plays a role in hormonal balance in both sexes. This test may be relevant for men who want insight into hormone metabolism, particularly when exploring overall hormonal balance or how hormones are processed in the body.

Customer reviews

4.8/5 (5)

• 5/5 — Great in-depth test. Simple collection process.
• 5/5 — This test gave me so much clarity on my hormone levels. Easy to do at home and the results were really detailed.
• 5/5 — Super convenient and the results were truly eye-opening. Worth every penny for understanding my body better.
• 5/5 — Sample collection was straightforward, and the turnaround time was as promised. Very happy with the information I received.
• 4/5 — The test provided useful insights into my estrogen metabolism. It was a bit more complex than I expected to understand all the markers, but overall a solid product.